FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653005
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTA2450.001241294717.7013442
TACGTTA2050.00930841617.6212889
CTCAACG2950.00396338714.8766171
CTCTACT3300.00833386913.2987961
GTATAAC4955.4975084E-513.1398946
CTCGAAG4250.003149471712.0471441
CTCCTAT5400.001513704410.8360551
CCCATCT5850.002944727210.0025131
CTTCTCT8355.8378377E-59.63565351
CTTTAAT11302.002149E-69.0620111
CTTTACT6500.00702153249.002261
AAACACA7350.00212254988.8493176
AGTATAA8700.0096859427.47614675
CCTTCTT12204.3325647E-47.19443041
GGATAAA11700.00219930086.87672761
ATTTAAT12050.00299860566.6769881
CTTTTGT11050.0096520096.61930941
CTTTTAT13950.00198626126.2919031
CTTTTTT26352.9680403E-55.2740951
Produced by FastQC (version 0.11.5)