FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459763
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTAA12901.1583865E-47.2989661
CTTCAAT37150.06.82365271
CTTTCGA23751.4158468E-76.4041771
CTTCAAA45200.06.24933531
TCAACGA24402.5066402E-76.21291072
CCCTTGT15258.9345244E-46.17420671
CCCATGA19105.5526725E-56.06728361
ATAAGTG16754.387023E-46.03142558
CTTCATT40951.8189894E-126.01357461
CTCCGCT20803.0006506E-55.91961241
CACTCTT20903.2290805E-55.89128881
CTTCCGT31051.8009814E-85.8315781
CTCAAAT19901.0058884E-45.82337241
CTCAGGA16700.00264870215.63812351
CCTCTCT15700.0082848115.5359131
CTCATCT17050.00338669755.5223841
CTCATAT18600.00158911165.4515851
CTCAAAC20207.689083E-45.37833171
ATTCGAA17700.0052638725.31958531
CTCAATC17750.00544115955.30460071