FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2511608
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAC7700.00324137638.4472851
CTTTAAT33100.07.20528751
CTTGGAT17351.8565861E-67.08132931
CTTCGAG15802.740983E-56.86119131
CTCCGCT20304.3623186E-76.7643011
CCCCAAT10750.00832954056.72290371
CTCCGTA15201.2236464E-46.656561
CCTACGA12100.00356129186.5701111
CCCTGCT29851.7644197E-106.53709551
CCTCGAC15802.0247081E-46.40377861
CTAAAAC14100.0025709676.15074161
CTCTGAT17851.4814278E-46.07321171
CCCGATT15500.00111539016.0614571
CCCGCAT19407.184569E-55.96051261
CTTCCGT28651.3447061E-75.8018781
CCCGAAC15100.00551773475.74340871
CCCTTAT17809.359337E-45.6842531
CTCGAAA15300.00638289245.66833071
CTATGCT15500.00736734225.5951911
CTTCTGA28557.6730976E-75.56906031