FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973122
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAG1800.0043225720.1263221
CCCACGT3250.0080238413.3762631
CTACGAA4705.1223073E-412.3327251
CACACGG5752.1750244E-411.3407451
CCCCAAT5250.001295850111.0407251
TCAACCT6900.00120213999.4200223
CCCACAT6950.00124662159.382631
TCAAACC7753.3462833E-49.3168167
GTGTTGT7000.00137352849.2835429
CTCCTGT11302.28104E-68.9766961
CTCAACA6500.00759385538.9175081
ATTTCGC7400.00227766048.7817286
CCCACGC6650.0091541748.7163621
AATTTCG9352.3048632E-48.4947435
CTCTATT8558.615062E-48.474241
CTCATTA7700.00316430478.4687381
CTCCGAA8750.00108349458.2805431
CCCGCTT8750.00108349458.2805431
CCAGGAT8300.00621964977.85653971
CTCTGCT8500.00769810667.671681
Produced by FastQC (version 0.11.5)