FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813214
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAT3300.00872628813.2070731
CTCAATT4907.1160716E-411.8594121
CTCAAAG8808.883708E-710.7307471
CTTTGAT14255.456968E-1210.7046811
CTCCTCT5650.002338056210.2851541
CAATTTA6050.0042648779.5649554
CTTCGAT7350.0020275368.8945591
CTATGCC6650.0089633468.7385151
CTCTGCT7500.0024357168.7166681
CTTCCGA7550.00258696458.6589421
CTTGAAT8407.042278E-48.6474881
TCAAAGC11151.904115E-58.4347122
CAAGGTC8300.00631040857.8435514
CTCATAA8400.0067663047.7827391
CAAAGCC13803.387571E-57.33833033
CAACAAG11903.528003E-47.32493161
CTCATTT9950.00373714027.30039261
AGTTTTC11350.00179628387.0104385
TTATTCC10350.0057122996.98888646
AAGATTG11750.00258784376.7717855
Produced by FastQC (version 0.11.5)