FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063287
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAA6202.2707973E-612.9141771
CAAAGAG13051.6520098E-68.3665511
CAACAAC15252.1272353E-78.1141831
CCCGCAT8100.0047941268.0876661
AGATCCG8150.0055587017.9556466
CACAAAT11251.803688E-47.76415971
CGATAAT11150.00139382997.18097731
CAACGGA10250.0048946297.10136561
CCCGAAA11400.00176252567.02351
CAACAAT15850.00133611265.9700751
CTTTAAA17000.00307887635.56621741
CTTATTT20408.190758E-45.35213231
CTTTGAT19250.00229553885.29374551
CATTGAT17950.00584436445.27162651
TCCAGTT18150.0075064565.160097
CAAAGAT19900.00394717145.06905273
CATAAAA23258.5543696E-45.00913521
Produced by FastQC (version 0.11.5)