FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533723
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCTA1500.001523468624.0839734
CTCAGAG1600.002078789522.831121
CTCCGAC4001.21715224E-414.6119171
GATTTAG3150.00664212213.7622715
TGTTATG4100.002636583512.3356937
CCCAAAG4200.00290596412.1765981
CCCGCTT4350.003778707511.7567161
GATCTTG5500.00195245110.509375
CTTTGTA7201.4143543E-410.1471641
CATCGAA6050.0039240459.6607721
TTCTACA6300.00600447559.1748476
ACAACAT6600.0087948538.7578083
CTTTGAA8450.00676816567.7814941
CTTTTTT17750.0048909575.3508431
Produced by FastQC (version 0.11.5)