FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1371767
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA3304.483366E-415.5995241
CGTAGCG4650.00681496210.8643687
CTCAGTT6959.279205E-510.5813831
CTATGTT9201.5872721E-48.7928981
CTTCCGA10901.165721E-58.7708991
CTTTAAT18105.5661076E-108.5323351
CTCCGAT8950.00117032118.2168271
AGGGATA9500.00253450877.596894
CTAAAAG10657.673871E-47.59574371
CAACTCT10859.357787E-47.455731
CTTCCAT24858.731149E-117.39845131
CTCAAAC11200.00131139087.22273831
CTCCAAG12656.1781117E-46.9761851
CTCACCA10850.0076935166.77793651
CTCTTGA13450.00124058456.5612441
CTTCATT21357.704857E-76.54459761
TCAGTTT16756.282216E-56.46302562
CTTTAAA19801.109882E-56.31409261
CTTCCGT15408.356548E-46.20797351
CTCCATT15408.356548E-46.20797351
Produced by FastQC (version 0.11.5)