FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953139
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT4900.038.1577769
CACACGT5150.034.9090967
ACACGTC5700.032.80238
AGAGCAC5700.031.5423243
GAGCACA5850.030.733554
GCACACG6600.028.329266
AGCACAC6450.027.8731525
GAAGAGC10450.017.2901731
AAGAGCA13750.013.0777862
TCTCGTA5850.07.633213535-39
CTTCGCC9850.00355876687.33735231
CTTCAAG11550.0021804956.88313531
GATCTCG5051.5224941E-96.84575635-39
ACGTCTG6550.06.80700710-14
CTCGTAT6403.6379788E-126.75216235-39
CAGTCAC6003.092282E-116.72653520-24
CACTGAC6207.094059E-116.504966725-29
ATCTCGT6659.094947E-126.49832135-39
ACTTCGA6452.910383E-116.47152830-34
TCGATCT6154.0017767E-106.31912930-34
Produced by FastQC (version 0.11.5)