FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371087
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTGG16102.4617676E-46.3080731
CTACGAC21003.3925502E-55.87251571
TACGACG22351.4494568E-55.83091972
GTCAGGA28356.2396066E-75.6294271
CTTGAAT33053.9016413E-75.26786141
CTCTATT23501.8593208E-45.247781
GTATACC18250.0073973285.1674351