FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592683
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGT1500.001542095824.0342966
CCCAAAT4200.002984233712.1338481
GTTTTGG5450.001841432310.583916
CTTTGAT9502.3006742E-69.9625281
CAAAGAC7351.8052627E-49.9051831
CTTAAAT6350.00602108759.1720431
CACAAAG7150.00154391939.1640251
ACAAAGA9850.00363668877.32009032
CGAAAAA10700.00739840746.80402761
Produced by FastQC (version 0.11.5)