FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944351
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAATT3850.001745370213.0336982
GTTTCGT6800.00106984479.5408291
CCGTATA7650.0032933298.4313364
CCTCCGT7800.00372290968.3176451
CTTTAAT8900.00134805798.099581
CGTTGAT9700.00314155737.4315741
GTTCAAA10150.00488857237.10209471
CTCAAAA11500.00214053046.89520741
CTTCAAT13900.00224990356.22327471
CTTTGAT15259.438437E-46.14505861
CTGCAAT14350.00321475956.02811961
GATATCC15650.00138460515.95217045
ATATCCG18352.4215298E-45.8573556
CATCGAC14950.0050725535.7861881
GGAATAT18700.00193796675.3653963
ATATCCA18750.00200817575.3502386
Produced by FastQC (version 0.11.5)