FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548012
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC3450.003011015715.502119145
CTATAAT5200.001253297711.08332351
TCAATCT6607.00719E-510.8755323
CCCGTTT6204.5984707E-410.4576511
TCGTAAA5550.002216730310.3502312
CTCCGTT5750.00289356910.0231781
CTTCACT5900.0035811469.7683531
CCAAAGT5950.0039458289.6544182
GGGGGGG1300.0078483258.882775100-104
CATGAAT6550.0084640748.7989751
CAACTTC7900.00433447448.1772993
CTTCAAT9150.0017795277.87339971
CTTTTAT11352.2453374E-47.61673361
CTTCATT11050.00141021927.17156271
CTTTCAA10600.00747731146.79637771
CTTCATC14350.00323015346.0243851
CTTTTTC18300.00137742545.51137971
GGCCATT4100.0041277355.0516562125-129
Produced by FastQC (version 0.11.5)