FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883567
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT10304.3291948E-1011.8542677
ACACGTC12507.2759576E-1211.4916678
CACGTCT11251.877197E-910.8509829
CTCTAAT9602.6460853E-48.3882831
CCCACGA16205.9179E-88.1340931
CTCAGTG9300.00178898677.8717021
CCCACTT15051.3891749E-67.78278731
AGCACAC20006.217306E-97.54140575
CTTATAA31350.07.47246841
GAGCACA21702.1907726E-76.61962374
CTCTTGA23559.86056E-86.5279981
CCCCTTC19157.1131726E-66.49877931
CTTCGAA18102.165639E-56.47132251
CTTCTAT29557.1122486E-106.44121031
AGAGCAC19008.467468E-66.4262613
CTATTAA13850.00182016366.34283071
GCACACG19301.0793814E-56.3263716
GTTCAGT15056.696051E-46.32351451
GTTCTAT21205.1518455E-66.2156751
CTTCAGT20251.6879656E-56.14575961
Produced by FastQC (version 0.11.5)