FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686267
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAA10851.5906015E-58.5577351
GTTCCGA13051.5857535E-47.11505271
CCTCGCT14801.0067769E-46.75634151
CTTCGGA18103.104409E-56.313741
GAACGAT14958.314824E-46.21079831
CTCAAAG17601.4339708E-46.08728841
CAACGAA16400.00251927345.6616731
CTCAAAA20852.3978317E-45.48099231
CTTTTGT25452.4886078E-55.33225731
GAGAATC20250.0010192735.2658832
CAAAGTT19050.00272348035.22256043
CAGCATA22407.1127625E-45.0760094
Produced by FastQC (version 0.11.5)