FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38058334
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA389840.10243222942969601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGA183200.017.817851
GACTAAC124000.017.7405571
CTCTACG213950.017.6790161
CAATGGA190850.017.5556348
ATGCGAT125150.017.4816191
GGGCATC156700.017.453931
ACTGATG107700.017.3603041
TGACAAC155750.017.2199731
TGCGGGT135200.016.981251
CGGACAC140850.016.9677011
AAACTAG126800.016.959533
CCCTCCT199500.016.8484541
CGCTTCA133700.016.8051361
TGACTAG123900.016.8021351
CTACCCA198550.016.7980691
CGTTGGG186550.016.7846681
GTCGTAA157550.016.769251
CTGATAG103000.016.7538871
TGTAAAC125600.016.7490797
CGACCTT141750.016.7329371
Produced by FastQC (version 0.11.5)