FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27092793
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG136050.018.3530711
CGGACGT128850.018.2761521
TTGGCAA226450.018.2669451
ATCCTCT131000.018.1942964
GTCGACT123200.018.1475418
CCCATAC171750.018.0038341
CAGCCGA150900.017.905981
TGATCCT100300.017.8803622
CGCTATC109550.017.8250261
AGCCTAA130300.017.7656731
ATAACGC118400.017.675541
ATCGAGT99750.017.6707781
CATGGCG116000.017.6704121
GCCGACA112950.017.6047043
CTGATCC136400.017.5945661
GATCCTC102100.017.56523
CCTATTA127500.017.535981
CATGACA130750.017.4749981
CGTCTTT93900.017.4741977
GGCTCGA96300.017.4208561
Produced by FastQC (version 0.11.5)