FastQCFastQC Report
Mon 12 Sep 2022
EGAF00005003548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49460984
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATGGG1368113027.660448486022844No Hit
GTCTCCTT1354662827.388512933749965No Hit
TCACATCA1044883221.12540260015854Illumina PCR Primer Index 1 (100% over 8bp)
AGTGGAAC1029650620.81743056304743No Hit
AGTGGCAC2114880.42758550861018046No Hit
TCACCTCA1044180.21111185333474156RNA PCR Primer, Index 40 (100% over 8bp)
TCCCATCA821740.1661390319286814No Hit
CAGCTGGG579690.1172014693439985No Hit
GGCTCCTT514210.1039627517317488No Hit
AGTGGACC507560.1026182576553673No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)