FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024069
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATT5801.1380853E-613.7325381
TTGTACG6400.0068618079.0284546
ACAACTC6500.00779248878.8895547
GAGCGGC9900.0037626117.295725
GTTTGAA14707.690198E-56.89599371
TTTGTCC12850.00669858236.182917
ATGGCGG14750.00427453365.87614638
CTTTTAT21352.7610845E-45.42635541
CTGAAAT17550.00476994835.363551
CAAAGAT17700.0052718675.3180961
CTGATTT19250.00245151235.26603131
CTTTATT20650.00103387925.2596561
AACAATT18000.0065842445.2182232
Produced by FastQC (version 0.11.5)