FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4804480
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGT25604.958529E-75.99123761
CCCCAAC25655.142483E-75.9795591
CTCACAA19959.253269E-55.8575271
GTCACGT16450.00200540485.77184531
TAGTCGA17600.00511222575.3331197
CTCTGAT30602.3680423E-65.2509571
TTCTAAG18100.0070989825.1857956
CCCCCAT24703.531073E-45.0267721
CTTACAT24703.531073E-45.0267721
Produced by FastQC (version 0.11.5)