FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666106
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCTC3100.005907101614.0087971
CCTTCCT6002.4353178E-512.0631321
CCCATCC4250.003407355611.9212121
CTCAATT4300.003718834611.7825931
CCCCCCC1008.928439E-411.54493530-34
AGTTCAA8155.5836164E-48.8508195
CTTTAAT14751.4477337E-88.8326651
CTTCTCT8256.096092E-48.7731871
AATCCAA8200.00580029757.91716535
CTTCCAG8250.0059421877.89586831
CCTTGAT8250.0059421877.89586831
CTTGTTT11101.6456423E-47.82473371
CTTAAAT9250.00189283927.82473331
CTCTTTA8500.0077642017.66363671
CGATTTT8500.0080028927.63773634
CTTTTAT19552.1911183E-77.03425551
CTTTAAA13702.4395445E-46.86805961
CTTTGAT11600.00224459786.86350541
CTTCAAT11750.0025696626.77588651
GCTTTTC11850.00291077336.6959995
Produced by FastQC (version 0.11.5)