FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519313
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGACGAATGATCTCGTATGCCGT8070.15539761184487969TruSeq Adapter, Index 6 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2350.062.5256588
CACACGT3050.052.763657
CACGTCT2800.052.476899
GCACACG3900.041.2678536
AGCACAC4300.037.428985
GAGCACA6350.025.345614
AGAGCAC6350.025.343173
GAAGAGC9400.017.9041251
AAGAGCA10600.016.5053442
GTTTAAC2650.002568121815.8772441
CCTTGGG3900.002303277412.5597815
AGTCACG2900.012.5449920-24
CAGTCAC2950.011.85804320-24
CTTCGCT4450.006077761811.0308191
CAACATT5700.0033648319.8420341
ACGTCTG3405.456968E-129.4656910-14
GTCACGA4100.09.21456620-24
CCAGTCA3951.8189894E-128.85600620-24
TCACGAC4200.08.66201825-29
CGACGAA4500.08.39549425-29
Produced by FastQC (version 0.11.5)