FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11204843
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT92800.06.43723151
CTCAGAT65700.06.0986061
CTTAGCT24601.0162403E-55.6266741
CTTCAAT148500.05.49445721
CCCAAGT34552.8163413E-85.48224641
CCCAGAA97200.05.47129151
CCCAAAC50600.05.47099541
CTCAATT67550.05.3923481
CTTAAAA140550.05.33875041
CTAAAAG56800.05.2585831
CTCAGGA68600.05.2036161
CCCTAAA26804.238358E-55.16478251
CTCCAAT82050.05.14970871
CTCCTGG65100.05.14766261
CTCAAAC87900.05.13849641
CTCAAAA153050.05.04551841
Produced by FastQC (version 0.11.5)