FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979856
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAAG4202.0375269E-413.7542836
CCCAAAT7000.00122312889.40218451
CTTACAT6400.006197279.1410131
CCCAGCT7700.00291025438.5474411
CTTCAAA16558.607094E-87.95350931
CCAAAGC12304.7761184E-47.1344491
CTTCTGT14656.45659E-56.9883511
CTTAAAA12757.2004966E-46.88264471
CAATTTC14707.9699144E-56.8771424
CTTTCAA17451.2796983E-56.70515541
CTTCAAT15701.5959068E-46.5209771
CATTTAT17157.245707E-56.39604331
CTCATCA12800.0056549366.28444621
CTGTAAT13100.0071928946.14052721
CAAAGAT18201.6400963E-46.02704141
CTTTCAT18251.7028103E-46.01052861
CTCAAAA14650.00345094785.9900151
CTTTAAT17150.00323260065.54323771
CTTCCAT17800.00501342165.3408161
ATTTTGT20409.1119605E-45.3095586
Produced by FastQC (version 0.11.5)