FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473202
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTACG1750.003771189620.6035966
CCCAAGA2155.1036326E-420.2012841
CTTCGCG2700.002353107316.0862081
CCTCATT3350.00985178412.9650041
CCCAAAG4907.315914E-411.81843951
CTTCAAT7051.2544435E-410.2677931
CTTTAAT5800.00298638049.9845441
TTCAATT7452.2674703E-49.6826112
CTTTGAT7550.0026651248.6290261
CTTTCAT8508.1828533E-48.5162281
TTGATTT9950.0039920537.24979353
CTTTTCT12054.2276722E-47.2087581
CAAAAAG12556.730756E-46.92155651
CTTTTTT17900.00601306325.25722461
CTTTTTC18300.00778689385.14231251
Produced by FastQC (version 0.11.5)