FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008269
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA8050.00413179548.2232861
CTCAAAC21552.910383E-118.1914861
CTCAGAT16305.2343967E-77.6711421
CAACGTT15501.6393346E-57.11800531
CCCAAAT21701.893568E-87.11800531
CCCACGA14555.482971E-57.07723761
CTTAAGG12605.900801E-47.0050211
CCCAGAT25206.7775545E-96.71314531
CTCAATT20955.5565215E-76.67065241
CTCAAGT13500.00129287626.538021
CCCATGC25951.2432793E-86.51912361
CTCAAAA40250.06.3958891
CTTCAAA53000.06.24504231
CTCCAGG17701.036832E-46.23328161
CTCAGAC13000.00626622056.2236921
TTAAACG14200.00281884616.1005043
CTCCAAG18351.7002987E-46.0124841
CCTCAAG23453.8412763E-65.95949551
CCCAAAC14850.00377063555.9436541
CTTACTG15100.004540625.8452491
Produced by FastQC (version 0.11.5)