FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033461
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4650.006127153511.0204921
CTCAAAT9501.8472929E-710.7884811
CTTTCGT8104.148805E-59.9417661
CTAAAAC6450.0065483179.0800071
CCCTGTT9450.00209091537.7468311
CTTAAAG9550.00231866217.66571241
CCCATAT9550.00231866217.66571241
CTCCCAT8700.0086411857.57319551
CTTAAAT10050.00382034927.28433281
CCTCAAT10050.00382034927.28433281
CTCAAAA14154.029475E-57.24314981
CTTCGAT10350.0050871257.0731931
CTTGATT11550.0019044446.9721481
TCTTCAG11550.0021879316.88105255
CTTCAAA18153.0686879E-66.8569061
ACCACAT10750.00734681366.81000471
CTTTATG12150.00324474886.6278441
CTTCAAT14805.454086E-46.4303931
CTTTTAC15257.837395E-46.24064351
CTTCTGT18803.7622834E-56.230431
Produced by FastQC (version 0.11.5)