FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957117
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGT1750.003798728320.5803496
CTCCCGT4753.230539E-513.9059831
CTCTGAT8607.777136E-59.3874361
CAAACGA7250.00162995699.1108171
CCCATAT8507.151603E-48.6344341
CTCATTT10456.397594E-47.72554641
CCAAAAG11702.5786026E-47.5274561
TAAAGCT10800.00111000687.33651353
CTTCAAT15151.2221924E-57.2666031
GTATAAA11550.00185393056.989781
CCCGAAA10650.0065483966.8913321
CTTTAAT13952.57131E-46.8394621
CCCTGCT13109.403608E-46.72299431
CAAAAGG12100.00302552486.6720631
CTCTTTT23707.2372677E-76.1934761
CATTCAT13100.0069280136.1627451
CTTATTT15750.00112105466.0578091
AATTGAT14750.00440514235.86016656
CTTTAAA15700.00714779555.60963251
CAACAAA17300.00343318755.51505761
Produced by FastQC (version 0.11.5)