FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053565
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA13402.401066E-99.808931
ATTTAAG6800.00105976219.5509316
CTCCCGA7350.00193382368.9414741
TTCGAAC10407.2762615E-47.6333062
GTTTGTT11550.00195624036.954481
CTTCAAC14103.1188794E-46.7325291
CGTTCAT12150.00333236836.61104971
CTTCCAG20102.2396216E-66.5392881
ACAATTT12400.00466212446.4021283
CTTCTGT13750.00172294586.3728331
CTTCGAT14000.00211028246.25903221
CTTTTTT31102.4265319E-96.1047371
TATCTGT13200.0089309996.01354939
TTCTGTA15400.0069565115.62359332
CTTTCAA17100.00317762985.5513831
AAATATA25351.1263162E-45.1242144
GATTTTC21200.00153478265.1058445
Produced by FastQC (version 0.11.5)