FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500230
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT1400.001086375325.5223223
GTCACTT2705.027754E-621.1740742
CTGAGGC1950.007071638518.480791
ACATAGA2800.003272174415.3103326
GCAGTCC4150.00891783313.161332145
GAATTGT5251.0582164E-412.2507144
CTGCAGA4250.003514092911.8711891
TTCGAAA5903.1321804E-410.9010592
CCCGAAT4650.006871810.8500131
AATTGTA4800.00927966910.4195325
CCCCGAA4850.00939201610.402591
CTTCCAT7151.5114083E-410.0804311
TCGAAAT7400.00249728058.6913853
CTTTATT8458.0581923E-48.5295951
CGTTTCC7950.00478088868.0884775
TTTCAAG8300.0070258057.7489462
CTTCTTT14756.302026E-46.352381
Produced by FastQC (version 0.11.5)