FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130269
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGTT11702.9012812E-58.1584431
CTCATGA16707.9081656E-77.4745011
CCCGAAT18354.55575E-77.2025491
CACGTCT12054.5222096E-47.16987139
CTCAACT13852.3472683E-46.89196971
CTTAAAT23005.9500962E-86.70411161
CCCACAT22102.044817E-76.64488551
CCCAAGA13400.00121201126.57546141
CTTTAAA33507.2759576E-126.57546141
CTCGGTT13600.00143309126.47876361
CTCAGAT17156.8711815E-56.4220981
TCTTACT14605.652077E-46.4126797
CTCAGAC12600.0046081586.4102051
CTCAAAT25052.9861985E-76.1554721
CCCAAAT14450.00283365256.09765961
CTCATGT14500.00294531566.07663351
CTCAAAG36604.2564352E-105.8179061
CCCATAT22901.708761E-55.7714751
CCCATTT28104.160811E-75.7486541
CTCAATT18200.00101513955.6481531
Produced by FastQC (version 0.11.5)