FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553105
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAGG4800.0082572210.5852281
CTCCTGT4850.00891663310.4761021
CCTTGAT7051.2213601E-410.2956631
CTCATTT5950.00360893579.759251
CATATTG6850.0011311519.4814645
GTTTGAA6900.00114643969.4675331
CTTTCAT8507.7088276E-59.3932781
ATCAGAT7954.0910265E-49.13011551
CTTTATT10458.039113E-69.0296421
CTTCAAC6450.0070158499.002721
GATTTCC10651.05227984E-48.1312095
AATATCT10900.00119808557.2826555
TCATATT10000.00417819577.21644834
TTCTCCA10050.0043861787.1805462
CTTCTTT13652.2505305E-46.9128021
TTGATAT10600.00735550336.807973
TTGATTT14756.2124204E-46.36025953
GATTTTG12650.0057317786.27517275
TCTTCTT13150.0085677096.03657257
TGATTTT17759.15434E-45.69184644
Produced by FastQC (version 0.11.5)