FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321694
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTAT15905.4169504E-98.6728621
CTTTACT9653.068367E-48.2731591
CTCATCT12407.807097E-68.1943081
CTCAAAA15051.5785336E-67.71595051
CTCATTT12306.2498664E-57.67086271
CCTCATA9800.00325161227.40593531
CTCAAAC11200.0015078227.12821251
CTTTAAA13251.5689748E-47.1208771
CTTGAAC12757.8532833E-46.83088641
CTATGAT12050.00325912036.62539241
TCGAAAT12150.00369493526.5464843
GTTTATT13650.00170073336.3804981
CACCGAA13850.00200337246.2883611
AAACATT16252.8885636E-46.22968864
CTTTAAT14400.00310024066.04818061
TCAGATT14900.00471892635.82441662
CACCAAC15350.0063081945.6738631
TATATTG15900.0096873235.4572755
TTAAAGA17300.0040892525.4344392
CATTGAT17400.0041965895.4225071
Produced by FastQC (version 0.11.5)