FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589075
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGTT4505.6601493E-817.8841131
CTCTAAT3203.6850196E-416.0042481
CTCTTCT8003.0364354E-610.9743421
CCCATTG4800.00778634310.6694991
CATCAAT11803.3760443E-89.920311
CTTCCAT11051.471406E-69.2694291
ATGGACA7300.00200247448.9059172
CTTCAAT10155.1122566E-58.6497281
CCCATTT7900.00364947468.3349431
CTTCCTT11753.1854797E-58.094551
TCAATAA10951.4369584E-47.91637133
CTTTAAT9250.00170167387.90943531
CTTTATT15301.7838265E-67.6509581
CTTTTAT15351.8735263E-67.62603571
TCAATGC9500.00250656797.6038833
CTTCTCT8800.0096096387.48250631
CTCCTTT12951.0678713E-47.3444761
CTTTCAT13151.2903988E-47.23277331
ATATTCC13201.5819797E-47.11423876
CTTTCAA10950.0088173036.6814871
Produced by FastQC (version 0.11.5)