FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813759
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4000.002202501312.6376317
CTCAGTT7251.151343E-511.1667491
CCCAAAT9001.2398908E-48.9954361
CCCAACG8607.821036E-48.5580261
CCCAGCG18458.0035534E-108.3771261
CCCAACA8900.00109895878.2695541
GATCCGA8550.00705725237.74726631
CCCACCT10556.882818E-47.6738321
CTCAAAT15552.0937496E-67.57289031
CCCAGAA15953.0641568E-67.3829741
CAAGTCT9950.0039623627.25779253
CCCCATC13701.993705E-46.98384951
ATCGATT10450.00637768166.91091062
CCCATAA14253.228236E-46.7142981
GTCCTCT11100.009514876.63054281
CTTTCAT32601.4188117E-106.3213891
CCCATGT14150.00217986646.24161341
GTCAAAT16552.9135027E-46.22591
TGTTTCG14000.00239352046.18986035
CCCTCCT13150.00700879476.1565731
Produced by FastQC (version 0.11.5)