FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868346
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAG4600.007087704710.8064071
GTTTACA7151.8438812E-49.88563256
CTCAAAA13002.4017936E-89.2863861
CCACATT6950.00155664049.1567922
CCTCAAA9100.00194512847.80368571
CAACACC8300.00744456057.70026351
CAATATT15301.7774844E-46.46691474
AAAGGTT12150.0046876196.3984814
CTTCAAG12300.00506766336.35080431
AACAAAG12850.0083423836.0530632
CGAAGAA15550.00142764855.93682341
ATAATCA15450.0090637385.48988538
AAAAGAT21805.229839E-45.18709233
Produced by FastQC (version 0.11.5)