FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558473
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGGT3100.005662765414.0977651
CTATGGA4050.002263277312.5893631
GCACAGG5601.7555692E-411.6034668
CTTATAT5450.001691861810.6918831
GAACTTC6250.0056580229.2415175
TATGAAG6300.0060412559.1681719
CTTATTG6450.00681761759.0342261
CTTTTAT9703.16054E-59.0109421
AAAGGCA8150.0054431757.9729344
CTCCTTT10050.0040064017.2476071
CTCTTTT12707.187473E-46.88237331
CTTCTTT15601.54182E-46.53678371
Produced by FastQC (version 0.11.5)