FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52050698
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTGAAACGTGTAGATCT3453030.6633974437768346Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC661750.079.965769
AGAGCGT696850.076.041958
GATCGGA717050.074.245891
ATCGGAA742950.071.758862
TCGGAAG748300.071.154593
AAGAGCG761050.069.8126757
CGGAAGA770750.069.091174
GAAGAGC1120700.047.6999786
GGAAGAG1568750.034.387445
CGCCGTA406950.026.38958455-59
TCGCCGT418600.025.5789555-59
GTCGCCG428000.025.09507255-59
GCCGTAT432200.024.77741255-59
TGGTCGC443050.024.20048950-54
GTGGTCG454650.023.7265250-54
CGTATCA456150.023.57516760-64
CCGTATC447300.023.49712660-64
GGTCGCC449500.023.45613955-59
TCTCGGT496750.021.9175145-49
CGGTGGT507450.021.32920850-54
Produced by FastQC (version 0.11.5)