FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70314552
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC4375530.6222794393968407TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA912850.082.403191
ATCGGAA960650.078.68622
TCGGAAG960850.077.8535463
CGGAAGA984250.075.383764
AGAGCAC1360750.053.9560668
GAGCACA1422200.051.4769139
GAAGAGC1456950.050.816416
GATCGGG94850.050.6600571
ATCGGGA102050.046.951692
AAGAGCA1854000.040.004037
GGAAGAG2028200.037.0542685
TCGGGAA143100.032.9315453
TATGCCG674650.025.04823145-49
CTCGTAT699250.024.18622240-44
TCTCGTA672200.023.83928940-44
ATGCCGT712200.023.77034645-49
CGATCAG713500.023.66865330-34
TGCCGTC710950.023.63265645-49
TCGTATG730500.023.18729240-44
ACGATCA761850.022.37397830-34
Produced by FastQC (version 0.11.5)