FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56195880
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC4858370.8645420269243937TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA963950.086.508361
ATCGGAA1008550.082.652442
TCGGAAG1003650.082.0636753
CGGAAGA1018450.080.2661364
GATCGGG85600.065.669271
AGAGCAC1318250.061.512898
ATCGGGA90800.060.468572
GAGCACA1371600.058.871899
GAAGAGC1411750.057.825326
AAGAGCA1719700.047.4904067
GGAAGAG1850200.044.5684745
TCGGGAA124650.043.3835453
CGGGAAG197000.026.6410184
TATGCCG728100.025.8576345-49
CTCGTAT746450.025.28095640-44
TCTCGTA717400.024.95042440-44
ATGCCGT757700.024.79964645-49
TGCCGTC760100.024.54583745-49
CGATCAG766600.024.52230-34
TCGTATG770700.024.44786340-44
Produced by FastQC (version 0.11.5)