FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21694067
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAAGATACATCTCGTAT327820.1511104395501314TruSeq Adapter, Index 5 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA254900.021.1226961
ATCGGAA257800.020.965832
TCGGAAG258450.020.8689043
CGGAAGA261400.020.4392594
TATGCCG73250.015.93470145-49
AGAGCAC363050.015.1355088
GAGCACA382400.014.3317159
CGTATGC81050.014.16862845-49
GAAGAGC402150.013.8803336
CGGCGAC14300.013.7563271
TCTCGTA83900.013.67098440-44
ATGCCGT84750.013.20788545-49
CTCGTAT86700.013.07895240-44
GCCGTCT91200.012.63961250-54
TCGTATG88200.012.263823545-49
TGCCGTC90800.011.91281650-54
ATCTCGT98450.011.79782840-44
CGTCTTC99450.011.76603850-54
CCGTCTT102800.011.28387650-54
AAGAGCA511000.010.9377427
Produced by FastQC (version 0.11.5)