FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62845192
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTGAAACGTGTAGATCT1749360.27836019659228667Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC660800.039.7257239
AGAGCGT709700.037.2132578
GATCGGA750550.035.1337361
ATCGGAA765850.034.6225052
TCGGAAG783200.033.7740143
AAGAGCG798450.033.185567
CGGAAGA802550.033.022934
GAAGAGC1199300.022.4261056
CGCCGTA282200.019.05056855-59
TCGCCGT301950.017.8525255-59
GCCGTAT309350.017.37860155-59
GTCGCCG328650.016.47715455-59
CCGTATC328150.016.14511160-64
CGTATCA335900.016.07907960-64
GGAAGAG1727400.015.9426055
TGGTCGC345650.015.67642450-54
GTGGTCG357150.015.2325550-54
GGTCGCC354900.014.94386455-59
TCTCGGT429650.012.799293545-49
TCGGTGG431200.012.46870750-54
Produced by FastQC (version 0.11.5)