FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60811715
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTTATATCTCGTAT1718160.28253766564550925TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA710250.040.9579731
ATCGGAA724250.040.1760642
TCGGAAG737800.039.262773
CGGAAGA754550.038.245124
AGAGCAC1052400.027.607468
GAGCACA1099400.026.3613769
GAAGAGC1148100.025.3567096
AAGAGCA1455550.020.28967
TATGCCG308150.020.02903645-49
TCTCGTA326150.018.39908840-44
CTCGTAT331000.018.27403840-44
CGTATGC329900.018.1767745-49
GGAAGAG1647750.018.0460935
ATGCCGT343450.017.52719145-49
GCCGTCT352450.016.8710150-54
TATCTCG344300.016.80594440-44
TCGTATG346950.016.60655245-49
TGCCGTC350650.016.32909450-54
ATCTCGT363100.016.23925640-44
CGTCTTC390800.015.89438350-54
Produced by FastQC (version 0.11.5)