FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58780002
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG155150.010.97230445-49
GATCGGA529300.010.2631361
ATCGGAA555250.09.9465692
TCGGAAG561550.09.8756163
CGTATGC176150.09.60660245-49
CGGAAGA578900.09.5170254
CTCGTAT175450.09.42965940-44
TCTCGTA171750.09.27822140-44
ATGCCGT189900.08.88047845-49
GCCGTCT202200.08.23252850-54
ATCTCGT210000.07.664211340-44
CGTCTTC241950.07.07777550-54
CCGTCTT251700.06.99947650-54
AGAGCAC845600.06.8496768
GAGCACA901500.06.46514949
GAAGAGC935800.06.25920966
GTATGCC307150.06.014512545-49
TCGTATG196050.06.00579845-49
TGCCGTC200750.05.879512350-54
AGATCGG526900.05.558912145
Produced by FastQC (version 0.11.5)