FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64477680
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC450550.014.5463149
CGCCGTA138050.014.47378355-59
GATCGGA526450.013.2526581
AGAGCGT502500.013.1573418
TCGCCGT155800.012.68520955-59
ATCGGAA561650.012.3294662
TCGGAAG560650.012.1807813
GCCGTAT172850.011.77787655-59
GTCGCCG171800.011.75701855-59
AAGAGCG578150.011.648897
CGGAAGA593650.011.4914614
TGGTCGC194350.010.87761650-54
GTGGTCG204100.010.42902850-54
CGTATCA199800.09.81174660-64
CGGTGTA216150.09.67336735-39
ACCGGTG237400.08.61814735-39
AGCGTCG389050.08.6092259
TCTCGGT265750.08.35345-49
CACCGGT238450.08.30655635-39
ATCGGGA77350.08.11411
Produced by FastQC (version 0.11.5)