FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51664409
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA68450.012.96406455-59
GAGCGTC246250.012.717369
GATCGGA280650.011.6709131
TCGCCGT78350.011.381494555-59
AGAGCGT277150.011.3517788
ATCGGAA302950.010.8412282
TCGGAAG312700.010.478313
AAGAGCG332700.09.7614487
GCCGTAT94350.09.65119255-59
CGGAAGA338200.09.5810824
GTCGCCG92100.09.46189255-59
TGGTCGC107450.08.69007250-54
GTGGTCG118650.07.75978950-54
CGGTGTA120150.07.724457735-39
CGTATCA118050.07.528564560-64
ACCGGTG132100.06.95982335-39
CACCGGT130250.06.83600635-39
CCGGTGT153050.06.19663935-39
AGCGTCG200700.06.1042019
TCTCGGT160500.06.0703245-49
Produced by FastQC (version 0.11.5)