FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005004468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57482938
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC227000.013.0608289
GATCGGA233650.012.80441
ATCGGAA262550.011.5424292
AGAGCGT262000.011.5377748
TCGGAAG283800.010.7289443
CGCCGTA88500.010.45202855-59
CGGAAGA313850.09.63252454
AAGAGCG325050.09.4120187
AGCGTCG176400.09.08169659
TCGCCGT104850.08.89130955-59
GCCGTAT117750.08.11423655-59
GTCGCCG124550.07.601382355-59
TGGTCGC143000.06.833416550-54
CGTATCA139500.06.808131760-64
GTGGTCG151250.06.393584750-54
AGGTCGT149350.06.28106835-39
GCGTCGT162850.05.555343610-14
GGTCGTG173450.05.533731535-39
CGTCGTG168400.05.38086410-14
TCTCGGT201400.05.0967645-49
Produced by FastQC (version 0.11.5)