FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59303700
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGAC261950.016.439782
ACGACGC50700.015.6721225-29
CGACGCT55050.015.06585625-29
CGTTGGA304300.014.1235621
CACGACG58250.014.03906520-24
TGGACTA352700.012.5386314
TCCGATC71150.011.47395535-39
TACACTC405600.010.9747349
ACTACAC406400.010.8639357
CTACACT416550.010.6340248
CCGATCT92550.09.16556235-39
TACACGA95150.09.06699220-24
TTGGACT511200.09.0055173
ACACGAC95600.08.94847820-24
TTCCGAT100000.08.6563230-34
GACGCTC103200.08.61263225-29
CTACACG112550.07.806963420-24
TATGCCG97500.07.435859745-49
CTTCCGA127250.07.246999330-34
ACGCTCT125700.07.08252625-29
Produced by FastQC (version 0.11.5)