FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56403708
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA56700.011.25193355-59
GAGCGTC283900.08.9888999
TCGCCGT71450.08.78705255-59
GCGTCGG37050.08.415553145
AGAGCGT320400.08.2363028
GATCGGA350550.07.6139561
GTCGCCG84000.07.595054655-59
GCCGTAT87950.07.43529555-59
ATCGGAA367700.07.23680832
AAGAGCG376850.07.0410277
TCGGAAG378100.06.99931343
CGGAAGA394050.06.62377264
TGGTCGC98900.06.597230450-54
CGTATCA107050.06.406691660-64
GTGGTCG112850.06.11576550-54
CGTCGTG209650.05.9834766145
AGCGTCG229050.05.793262145
AGATCGG364300.05.7323895145
CCGTATC102500.05.38964960-64
GGTCGCC103250.05.36451955-59
Produced by FastQC (version 0.11.5)