FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005004543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005004543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59798058
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG175500.012.63263545-49
CTCGTAT196100.011.57150940-44
ATGCCGT209200.010.73626745-49
TCTCGTA191050.010.65548940-44
CGTATGC194600.010.26762445-49
ATCGGAA589150.09.260311
TCGGAAG598200.09.1171212
GCCGTCT222900.08.86022250-54
CGTCTTC258050.08.76031750-54
CGGAAGA624550.08.7527623
CGACACT256250.08.67969730-34
TCCGATC49400.08.6589673
GTCACGA279500.08.24813625-29
ATCTCGT227850.08.12634440-44
GATCGGA542300.08.0294261
CCGTCTT263950.07.74595550-54
ACGACAC263500.07.72555230-34
CATCTCG265250.07.035240-44
AGTCACG321900.07.004060325-29
TCGTATG215800.06.88021240-44
Produced by FastQC (version 0.11.5)